H00001836-M04

antibody from Abnova Corporation

Targeting: SLC26A2 DTD, DTDST

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001836-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001836-M04, RRID:AB_1137455

Product name

SLC26A2 monoclonal antibody (M04), clone 3F6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SLC26A2.

Antigen sequence

MSSESKEQHNVSPRDSAEGNDSYPSGIHLELQRES
STDFKQFETNDQCRPYHRILIERQEKSDTNFKEFV
IKKLQKNCQCSPAKAKNMILGFLPVLQWLPKYDLK
KNI


Isotype

IgG

Antibody clone number

3F6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Epigenetic silencing of the sulfate transporter gene DTDST induces sialyl Lewisx expression and accelerates proliferation of colon cancer cells.

Yusa A, Miyazaki K, Kimura N, Izawa M, Kannagi R
Cancer research 2010 May 15;70(10):4064-73

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SLC26A2 expression in transfected 293T cell line by SLC26A2 monoclonal antibody (M04), clone 3F6.Lane 1: SLC26A2 transfected lysate(81.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SLC26A2 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol