MA5-29417

antibody from Invitrogen Antibodies

Targeting: MESD BOCA, KIAA0081, MESDC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29417

Provider

Invitrogen Antibodies

Product name

MESDC2 Recombinant Rabbit Monoclonal Antibody (502)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

502

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MESDC2 in Lane A: HEK293 Whole Cell Lysate (30 µg). Samples were probed using a MESDC2 Monoclonal Antibody (Product # MA5-29417) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:26 kDa. Observed band size:26 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MESDC2 Recombinant Rabbit Monoclonal Antibody (502) (Product # MA5-29417) at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Mesdc2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with MESDC2 Recombinant Rabbit Monoclonal Antibody (502) (Product # MA5-29417, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MESDC2 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate, Lane C: 0.5 mg NIH-3T3 Whole Cell Lysate 2 µL with MESDC2 Recombinant Rabbit Monoclonal Antibody (502) (Product # MA5-29417) and 15 µL of 50 % Protein G agarose. Primary antibody: MESDC2 Recombinant Rabbit Monoclonal Antibody (502), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.