100-401-E79

antibody from Rockland Immunochemicals, Inc.

Targeting: AHSP EDRF, ERAF

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-E79

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-E79, RRID:AB_2610742

Product name

Anti-AHSP (RABBIT) Antibody - 100-401-E79

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Requirement for antiapoptotic MCL-1 during early erythropoiesis.

Turnis ME, Kaminska E, Smith KH, Kartchner BJ, Vogel P, Laxton JD, Ashmun RA, Ney PA, Opferman JT
Blood 2021 Apr 8;137(14):1945-1958

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α-Hemoglobin-stabilizing protein is a sensitive and specific marker of erythroid precursors.

Raess PW, Paessler ME, Bagg A, Weiss MJ, Choi JK
The American journal of surgical pathology 2012 Oct;36(10):1538-47

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Analysis of alpha hemoglobin stabilizing protein overexpression in murine β-thalassemia.

Nasimuzzaman M, Khandros E, Wang X, Kong Y, Zhao H, Weiss D, Rivella S, Weiss MJ, Persons DA
American journal of hematology 2010 Oct;85(10):820-2

Immunohistochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A. Normal bone marrow, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. C. AHSP stains nucleated EPs, but not mature, anucleate RBCs. D. CD71 stains nucleated EPs, but not mature, anucleate RBCs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10,000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max™ – fully automated for IHC.

Validation comment

Immunohistochemistry