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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00001936-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001936-M04, RRID:AB_1111803
- Product name
- EEF1D monoclonal antibody (M04), clone 4B12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant EEF1D.
- Antigen sequence
MRSGKASCTLETVWEDKHKYEEAERRFYEHEATQA
AASAQQLPAEGPAMNGPGQDDPEDADEAEAPDGGS
RRDPRKSQDSRKPLQKKRKRS- Isotype
- IgG
- Antibody clone number
- 4B12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged EEF1D is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
