LS-B10046

antibody from LSBio

Targeting: SSB La, La/SSB, LARP3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B10046

Provider

LSBio

Product name

IHC-plus™ SSB / La Antibody (clone 1E11) LS-B10046

Antibody type

Monoclonal

Description

Protein A/G purified

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

1E11

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation(IP) of SSB by using monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SSB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation(IP) of SSB by using monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SSB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-SSB / La antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.