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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunoprecipitation [2]
- Flow cytometry [1]
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- Product number
- TA500406 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500406, RRID:AB_2239866
- Product name
- Anti-SSB mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)
- Antibody type
- Monoclonal
- Description
- Anti-SSB mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SSB
- Isotype
- IgG
- Antibody clone number
- OTI1E11
- Vial size
- 100 µl
- Concentration
- 2.8 mg/ml
Submitted references RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP.
Muniz L, Egloff S, Kiss T
Nucleic acids research 2013 Apr;41(8):4686-98
Nucleic acids research 2013 Apr;41(8):4686-98
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SSB monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SSB monoclonal antibody. (1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody (TA500406).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SSB mouse monoclonal antibody (TA500406) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB(RC205013).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunoprecipitation of SSB(La) protein level by using anti-SSB antibody in HeLa cells.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody(TA500406),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
