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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunoprecipitation [1]
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- Product number
- NBP1-21395 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-21395, RRID:AB_1660058
- Product name
- Rabbit Polyclonal CLASP2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Clasp2 ensures mitotic fidelity and prevents differentiation of epidermal keratinocytes.
Identification of a role for CLASP2 in insulin action.
Shahbazi MN, Peña-Jimenez D, Antonucci F, Drosten M, Perez-Moreno M
Journal of cell science 2017 Feb 15;130(4):683-688
Journal of cell science 2017 Feb 15;130(4):683-688
Identification of a role for CLASP2 in insulin action.
Langlais P, Dillon JL, Mengos A, Baluch DP, Ardebili R, Miranda DN, Xie X, Heckmann BL, Liu J, Mandarino LJ
The Journal of biological chemistry 2012 Nov 9;287(46):39245-53
The Journal of biological chemistry 2012 Nov 9;287(46):39245-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLASP2 Antibody [NBP1-21395] - Samples: Wholecell lysate (50 ug) from NIH3T3, TCMK-1, and 4T1 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLASP2 Antibody [NBP1-21395] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CLASP2 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CLASP2 was also immunoprecipitated by rabbit anti-CLASP2 antibody NBP1-21394, which recognizes an upstream epitope
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLASP2 Antibody [NBP1-21395] - Detection of Human CLASP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLASP2 Antibody [NBP1-21395] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CLASP2 Antibody [NBP1-21395] - Detection of human CLASP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 (lot NBP1-21395-2) used for IP at 6 ug per reaction. CLASP2 was also immunoprecipitated by a previous lot of this antibody (NBP1-21395-1) and rabbit anti-CLASP2 antibody NBP1-21394. For blotting immunoprecipitated CLASP2, NBP1-21395 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
