22524-1-AP

antibody from Invitrogen Antibodies

Targeting: SLC6A3 DAT, DAT1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

22524-1-AP

Provider

Invitrogen Antibodies

Product name

DAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSKSKCSVG LMSSVVAPAK EPNAVGPKEV ELILVKEQNG VQLTSSTLTN PRQSPVEAQD RET (1-62 aa encoded by BC133003)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 22524-1-AP ( DAT antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22524-1-AP (DAT antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 22524-1-AP (DAT antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 22524-1-AP ( DAT antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 22524-1-AP ( DAT antibody at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using (Product # 22524-1-AP) (DAT antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DAT (IP:22524-1-AP, 4ug; Detection:22524-1-AP 1:600) with mouse brain tissue lysate 4000ug.