AP09326PU-N

antibody from Acris Antibodies GmbH

Targeting: MYL6 ESMLC, MLC1SM, MLC3NM

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09326PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09326PU-N, RRID:AB_2035919

Product name

anti MYL6 (12-27)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 12-27 of Human Myosin light chain protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.71 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2). Each lane was loaded with 35 µg of lysate. Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein. After blocking with 5% NGS and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800(TM) conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.