ADI-905-740-100

antibody from Enzo Life Sciences

Targeting: DRD2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ADI-905-740-100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-905-740-100, RRID:AB_2039067

Product name

Dopamine receptor D2 polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 μg

Storage

-20°C

Handling

Keep on ice at all times.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis: MW marker (1) and 10µg of rat heart membrane extract (2) probed with Dopamine Receptor (D2) pAb at 4.0µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis: Fluorescent confocal photomicrograph of rat cerebral cortex. Neurons are labeled with Dopamine Receptor (D2) pAb in conjunction with Cy3 conjugated rabbit IgG antibody (H+L).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Cellular ELISA: HEK293 cells expressing dopamine receptor (D2) were treated with indicated concentrations of agonist (Quinpirol) and antagonist (Siperone) and probed with Dopamine Receptor (D2) pAb (1:1000 of a stock solution of 1µg/µL) by cellular ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ±SEM (n=3).