ABIN453487

antibody from antibodies-online

Targeting: ZWINT KNTC2AP, SIP30, Zwint1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN453487

Provider

antibodies-online

Product name

anti-ZW10 Interactor (ZWINT) (Middle Region) antibody

Antibody type

Polyclonal

Description

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reactivity

Human, Mouse

Host

Rabbit

Epitope

Middle Region

Vial size

0.4 mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint.

Famulski JK, Vos L, Sun X, Chan G
The Journal of cell biology 2008 Feb 11;180(3):507-20

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ZW10 links mitotic checkpoint signaling to the structural kinetochore.

Kops GJ, Kim Y, Weaver BA, Mao Y, McLeod I, Yates JR 3rd, Tagaya M, Cleveland DW
The Journal of cell biology 2005 Apr 11;169(1):49-60

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Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling.

Wang H, Hu X, Ding X, Dou Z, Yang Z, Shaw AW, Teng M, Cleveland DW, Goldberg ML, Niu L, Yao X
The Journal of biological chemistry 2004 Dec 24;279(52):54590-8

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS