Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARG63418 - Provider product page
- Provider
- Arigo
- Product name
- anti-VPS35 / MEM3 antibody
- Antibody type
- Polyclonal
- Antigen
- C-SPESEGPIYEGLIL
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Human Brain Cerebellum lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63418 anti-VPS35 / MEM3 antibody at 0.05 µg/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: PFA-fixed and saponin permeabilized HEK293 stained with ARG63418 anti-VPS35 / MEM3 antibody and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: PFA-fixed and saponin permeabilized SHSY5Y stained with ARG63418 anti-VPS35 / MEM3 antibody and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin embedded Human Testis A) Epithelial cells of the epididymis and B) Some leydig cells stained with ARG63318 anti-CSN2 / TRIP15 antibody (Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3µg/ml or B)Citrate buffer pH6 at 10ug/ml.