Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-1397 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1397, RRID:AB_2257186
- Product name
- Goat Polyclonal VPS35 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
- Reactivity
- Human, Mouse, Canine, Chicken/Avian
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport.
A Discrete Presynaptic Vesicle Cycle for Neuromodulator Receptors.
Insulin-responsive amino peptidase follows the Glut4 pathway but is dispensable for the formation and translocation of insulin-responsive vesicles.
Retromer has a selective function in cargo sorting via endosome transport carriers.
The Dopamine Transporter Recycles via a Retromer-Dependent Postendocytic Mechanism: Tracking Studies Using a Novel Fluorophore-Coupling Approach.
Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction.
Structural basis for the hijacking of endosomal sorting nexin proteins by Chlamydia trachomatis.
An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells.
Functional characterization of retromer in GLUT4 storage vesicle formation and adipocyte differentiation.
A role for retromer in hepatitis C virus replication.
Vps35-dependent recycling of Trem2 regulates microglial function.
VPS29-VPS35 intermediate of retromer is stable and may be involved in the retromer complex assembly process.
Clinical and cellular roles for TDP1 and TOP1 in modulating colorectal cancer response to irinotecan.
Intracellular Transport of Vaccinia Virus in HeLa Cells Requires WASH-VPEF/FAM21-Retromer Complexes and Recycling Molecules Rab11 and Rab22.
Retromer disruption promotes amyloidogenic APP processing.
Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron transporter DMT1.
GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer.
The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.
Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21.
Han J, Goldstein LA, Hou W, Watkins SC, Rabinowich H
Autophagy 2021 Jun;17(6):1393-1409
Autophagy 2021 Jun;17(6):1393-1409
A Discrete Presynaptic Vesicle Cycle for Neuromodulator Receptors.
Jullié D, Stoeber M, Sibarita JB, Zieger HL, Bartol TM, Arttamangkul S, Sejnowski TJ, Hosy E, von Zastrow M
Neuron 2020 Feb 19;105(4):663-677.e8
Neuron 2020 Feb 19;105(4):663-677.e8
Insulin-responsive amino peptidase follows the Glut4 pathway but is dispensable for the formation and translocation of insulin-responsive vesicles.
Pan X, Meriin A, Huang G, Kandror KV
Molecular biology of the cell 2019 Jun 1;30(12):1536-1543
Molecular biology of the cell 2019 Jun 1;30(12):1536-1543
Retromer has a selective function in cargo sorting via endosome transport carriers.
Cui Y, Carosi JM, Yang Z, Ariotti N, Kerr MC, Parton RG, Sargeant TJ, Teasdale RD
The Journal of cell biology 2019 Feb 4;218(2):615-631
The Journal of cell biology 2019 Feb 4;218(2):615-631
The Dopamine Transporter Recycles via a Retromer-Dependent Postendocytic Mechanism: Tracking Studies Using a Novel Fluorophore-Coupling Approach.
Wu S, Fagan RR, Uttamapinant C, Lifshitz LM, Fogarty KE, Ting AY, Melikian HE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 27;37(39):9438-9452
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 27;37(39):9438-9452
Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction.
Elwell CA, Czudnochowski N, von Dollen J, Johnson JR, Nakagawa R, Mirrashidi K, Krogan NJ, Engel JN, Rosenberg OS
eLife 2017 Mar 2;6
eLife 2017 Mar 2;6
Structural basis for the hijacking of endosomal sorting nexin proteins by Chlamydia trachomatis.
Paul B, Kim HS, Kerr MC, Huston WM, Teasdale RD, Collins BM
eLife 2017 Feb 22;6
eLife 2017 Feb 22;6
An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells.
Lobingier BT, Hüttenhain R, Eichel K, Miller KB, Ting AY, von Zastrow M, Krogan NJ
Cell 2017 Apr 6;169(2):350-360.e12
Cell 2017 Apr 6;169(2):350-360.e12
Functional characterization of retromer in GLUT4 storage vesicle formation and adipocyte differentiation.
Yang Z, Hong LK, Follett J, Wabitsch M, Hamilton NA, Collins BM, Bugarcic A, Teasdale RD
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Mar;30(3):1037-50
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Mar;30(3):1037-50
A role for retromer in hepatitis C virus replication.
Yin P, Hong Z, Yang X, Chung RT, Zhang L
Cellular and molecular life sciences : CMLS 2016 Feb;73(4):869-81
Cellular and molecular life sciences : CMLS 2016 Feb;73(4):869-81
Vps35-dependent recycling of Trem2 regulates microglial function.
Yin J, Liu X, He Q, Zhou L, Yuan Z, Zhao S
Traffic (Copenhagen, Denmark) 2016 Dec;17(12):1286-1296
Traffic (Copenhagen, Denmark) 2016 Dec;17(12):1286-1296
VPS29-VPS35 intermediate of retromer is stable and may be involved in the retromer complex assembly process.
Fuse A, Furuya N, Kakuta S, Inose A, Sato M, Koike M, Saiki S, Hattori N
FEBS letters 2015 Jun 4;589(13):1430-6
FEBS letters 2015 Jun 4;589(13):1430-6
Clinical and cellular roles for TDP1 and TOP1 in modulating colorectal cancer response to irinotecan.
Meisenberg C, Gilbert DC, Chalmers A, Haley V, Gollins S, Ward SE, El-Khamisy SF
Molecular cancer therapeutics 2015 Feb;14(2):575-85
Molecular cancer therapeutics 2015 Feb;14(2):575-85
Intracellular Transport of Vaccinia Virus in HeLa Cells Requires WASH-VPEF/FAM21-Retromer Complexes and Recycling Molecules Rab11 and Rab22.
Hsiao JC, Chu LW, Lo YT, Lee SP, Chen TJ, Huang CY, Ping YH, Chang W
Journal of virology 2015 Aug;89(16):8365-82
Journal of virology 2015 Aug;89(16):8365-82
Retromer disruption promotes amyloidogenic APP processing.
Sullivan CP, Jay AG, Stack EC, Pakaluk M, Wadlinger E, Fine RE, Wells JM, Morin PJ
Neurobiology of disease 2011 Aug;43(2):338-45
Neurobiology of disease 2011 Aug;43(2):338-45
Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron transporter DMT1.
Tabuchi M, Yanatori I, Kawai Y, Kishi F
Journal of cell science 2010 Mar 1;123(Pt 5):756-66
Journal of cell science 2010 Mar 1;123(Pt 5):756-66
GOLPH3 modulates mTOR signalling and rapamycin sensitivity in cancer.
Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L
Nature 2009 Jun 25;459(7250):1085-90
Nature 2009 Jun 25;459(7250):1085-90
The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.
Hong Z, Yang Y, Zhang C, Niu Y, Li K, Zhao X, Liu JJ
Cell research 2009 Dec;19(12):1334-49
Cell research 2009 Dec;19(12):1334-49
Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21.
Zhang P, Yu L, Gao J, Fu Q, Dai F, Zhao Y, Zheng L, Zhao S
Genomics 2000 Dec 1;70(2):253-7
Genomics 2000 Dec 1;70(2):253-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human Cerebellum (A) and Mouse Brain (B) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VPS35 Antibody [NB100-1397] - HEK293T cell lysate. WB image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VPS35 Antibody [NB100-1397] - 10 ug cell lysate per lane of various cell lines as indicated, immunoblotted with vps35 antibody at 1:2000 at 4C for 20 hrs. Secondary antibody donkey anti-goat HRP at 1:5000 for 1 hr at room temp. WB image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.