NBP2-13517

antibody from Novus Biologicals

Targeting: VPS35 FLJ10752, MEM3, PARK17

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-13517

Provider

Novus Biologicals

Product name

Rabbit Polyclonal VPS35 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat VPS35 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in human cell line HEK 293.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human skin shows moderate cytoplasmic positivity in keratinocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human skeletal muscle shows very weak positivity in myocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human substantia nigra shows moderate cytoplasmic positivity in neurons and fibers.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining in human skin and skeletal muscle tissues . Corresponding VPS35 RNA-seq data are presented for the same tissues.