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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX112287 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112287, RRID:AB_1950143
- Product name
- CRMP5 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A case of paraneoplastic optic neuropathy and outer retinitis positive for autoantibodies against collapsin response mediator protein-5, recoverin, and α-enolase.
Saito M, Saito W, Kanda A, Ohguro H, Ishida S
BMC ophthalmology 2014 Jan 16;14:5
BMC ophthalmology 2014 Jan 16;14:5
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX112287 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 B: Raji 7.5% SDS PAGE GTX112287 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (5 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CRMP5 antibody [N1C1] detects CRMP5 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: CRMP5 protein stained by CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CRMP5(GTX112287) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CRMP5 antibody [N1C1] detects CRMP5 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500.
