PA5-21629

antibody from Invitrogen Antibodies

Targeting: IPMK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21629

Provider

Invitrogen Antibodies

Product name

IPMK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji, mouse brain.

Concentration

1.34 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IPMK using 30 µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IPMK polyclonal antibody (Product # PA5-21629) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IPMK Polyclonal Antibody (Product # PA5-21629). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. IPMK Polyclonal Antibody (Product # PA5-21629) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IPMK Polyclonal Antibody (Product # PA5-21629). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK Polyclonal Antibody (Product # PA5-21629) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IPMK Polyclonal Antibody (Product # PA5-21629). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK Polyclonal Antibody (Product # PA5-21629) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of IPMK in paraformaldehyde-fixed HeLa cells using an IPMK polyclonal antibody (Product # PA5-21629) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IPMK antibody (Product # PA5-21629) at 1:200 dilution.