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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AP5990c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP5990c, RRID:AB_10821041
- Product name
- FKBP8 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- FKBP8 Antibody (Center) (Cat. #AP5990c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FKBP8 antibody detected the FKBP8 protein (arrow).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "With HepG2 cell line lysate, the resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody FKBP38 ( abgent, Cat. AP5990C, 1:1000, 4°C ÿovernight ) and horseradish peroxidase conjugated second antibody. After washing, the bound antibody complex was detected using an ECL chemiluminescence reagentand XAR film (Kodak)."
- Primary Ab dilution
- 1:1000
