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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-401-A58 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-A58, RRID:AB_2103433
- Product name
- Anti-FKBP8 (RABBIT) Antibody - 200-401-A58
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 µg of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4° C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.
- Validation comment
- Western Blot
