GTX629886

antibody from GeneTex

Targeting: PUF60 FIR, RoBPI, SIAHBP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX629886

Provider

GeneTex

Product name

PUF60 antibody [GT677]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract7.5% SDS-PAGEPUF60 antibody [GT677] (GTX629886) dilution: 1:5000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.A. 50 ?g mouse brain lysate/extract7.5% SDS-PAGEPUF60 antibody [GT677] (GTX629886) dilution: 1:1000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PUF60 antibody [GT677] detects PUF60 protein by western blot analysis.A. 50 ?g rat brain lysate/extract7.5% SDS-PAGEPUF60 antibody [GT677] (GTX629886) dilution: 1:1000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PUF60 antibody [GT677]PUF60 antibody [GT677] detects PUF60 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PUF60 protein stained by PUF60 antibody [GT677] (GTX629886) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PUF60 antibody [GT677] detects PUF60 protein at on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. PUF60 antibody [GT677] (GTX629886) dilution: 1:100.