NBP1-49906

antibody from Novus Biologicals

Targeting: PUF60 FIR, RoBPI, SIAHBP1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-49906

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-49906, RRID:AB_10012096

Product name

Rabbit Polyclonal PUF60 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PUF60 Antibody [NBP1-49906] - Detection of Human PUF60 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize upstream epitopes. For blotting immunoprecipitated PUF60 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: PUF60 Antibody [NBP1-49906] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PUF60 used at a dilution of 1:200 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit