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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-27605 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PUF60 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431. Predicted reactivity: Mouse (99%), Rat (92%), Chicken (92%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PUF60 Polyclonal Antibody (Product # PA5-27605). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. PUF60 Polyclonal Antibody (Product # PA5-27605) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PUF60 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PUF60 polyclonal antibody (Product # PA5-27605) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PUF60 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 Polyclonal Antibody (Product # PA5-27605) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUF60 Polyclonal Antibody detects PUF60 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 Polyclonal Antibody (Product # PA5-27605) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PUF60 Polyclonal Antibody detects PUF60 protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. PUF60 Polyclonal Antibody (Product # PA5-27605) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
