Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00009244-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009244-M01, RRID:AB_537995
- Product name
- Mouse Monoclonal CRLF1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CRLF1 - cytokine receptor-like factor 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 over-expressed 293 cell line, cotransfected with CRLF1 Validated Chimera RNAi ( Cat # H00009244-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRLF1 monoclonal antibody (M01), clone 4F4 (Cat # H00009244-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 expression in transfected 293T cell line by CRLF1 monoclonal antibody (M01), clone 4F4.Lane 1: CRLF1 transfected lysate(46.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - CRLF1 monoclonal antibody (M01), clone 4F4 Analysis of CRLF1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CRLF1 Antibody (4F4) [H00009244-M01] - Detection limit for recombinant GST tagged CRLF1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 transfected lysate using anti-CRLF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CRLF1 MaxPab rabbit polyclonal antibody.