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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Other assay [1]
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Validation data
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- Product number
- H00009244-M01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRLF1 Monoclonal Antibody (4F4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: PEKPVNISCW SKNMKDLTCR WTPGAHGETF LHTNYSLKYK LRWYGQDNTC EEYHTVGPHS CHIPKDLALF TPYEIWVEAT NRLGSARSDV LTLDIL
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4F4
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CRLF1 expression in transfected 293T cell line by CRLF1 monoclonal antibody (M01), clone 4F4.Lane 1: CRLF1 transfected lysate(46.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRLF1 over-expressed 293 cell line, cotransfected with CRLF1 Validated Chimera RNAi (Cat # H00009244-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRLF1 monoclonal antibody (M01), clone 4F4 (Cat # H00009244-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CRLF1 expression in transfected 293T cell line by CRLF1 monoclonal antibody (M01), clone 4F4.Lane 1: CRLF1 transfected lysate(46.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CRLF1 monoclonal antibody (M01), clone 4F4 Western Blot analysis of CRLF1 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CRLF1 is approximately 0.03 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CRLF1 transfected lysate using anti-CRLF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CRLF1 MaxPab rabbit polyclonal antibody.
