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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM32654PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti PCLO
- Antibody type
- Monoclonal
- Antigen
- GST-tagged recombinant protein.
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- 4G3.3
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis (Representative lot data): Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with ant-Piccolo, clone 4G3.3 (1 μg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescent detection system. Arrow indicates Piccolo (~547 kDa).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry on Paraffin Sections (Representative images from a previous lot): Piccolo staining pattern/morphology on Mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1/300, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).
