MA5-27629

antibody from Invitrogen Antibodies

Targeting: PCLO ACZ, DKFZp779G1236, KIAA0559

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27629

Provider

Invitrogen Antibodies

Product name

PCLO Monoclonal Antibody (6H9-B6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

6H9-B6

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PCLO in rat and mouse brain lysates. The sample was incubated with PCLO monoclonal antibody (Product # MA5-27629) using a dilution of 1:1000. Samples were arranged as follows: 1) Mouse brain lysate, 2) Rat brain lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PCLO in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with PCLO monoclonal antibody (Product # MA5-27629) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Piccolo Antibody, D) Merged image. Magnification: 60x.