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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MA5-28620 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCLO Monoclonal Antibody (PCLO-01)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- The mouse monoclonal antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytomatrix of the neurons.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PCLO-01
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Peptidome characterization of ovarian cancer serum and the identification of tumor suppressive peptide ZYX(36-58).
Wang X, Liu G, Sheng N, Zhang M, Pan X, Liu S, Huang K, Cong Y, Xu Q, Jia X, Xu J
Annals of translational medicine 2020 Aug;8(15):925
Annals of translational medicine 2020 Aug;8(15):925
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blotting of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by Monoclonal antibody PCLO-01 (Product # MA5-28620) and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.
