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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-89018 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89018, RRID:AB_11041057
- Product name
- Rabbit Polyclonal MED15 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat MED15 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Activated transcription via mammalian amino acid response elements does not require enhanced recruitment of the Mediator complex.
Thiaville MM, Dudenhausen EE, Awad KS, Gjymishka A, Zhong C, Kilberg MS
Nucleic acids research 2008 Oct;36(17):5571-80
Nucleic acids research 2008 Oct;36(17):5571-80
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED15 Antibody [NBP1-89018] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MED15 Antibody [NBP1-89018] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MED15 Antibody [NBP1-89018] - Staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.
