Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN951610 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Coatomer Protein Complex, Subunit epsilon (COPE) (C-Term), (AA 280-308) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 280-308 amino acids from the C-terminal region of human COPE
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 280-308
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references New genetic associations detected in a host response study to hepatitis B vaccine.
Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex.
Ubiquitylation of epsilon-COP by PIRH2 and regulation of the secretion of PSA.
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML, Seielstad M
Genes and immunity 2010 Apr;11(3):232-8
Genes and immunity 2010 Apr;11(3):232-8
Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex.
Morikawa RK, Aoki J, Kano F, Murata M, Yamamoto A, Tsujimoto M, Arai H
The Journal of biological chemistry 2009 Sep 25;284(39):26620-30
The Journal of biological chemistry 2009 Sep 25;284(39):26620-30
Ubiquitylation of epsilon-COP by PIRH2 and regulation of the secretion of PSA.
Maruyama S, Miyajima N, Bohgaki M, Tsukiyama T, Shigemura M, Nonomura K, Hatakeyama S
Molecular and cellular biochemistry 2008 Jan;307(1-2):73-82
Molecular and cellular biochemistry 2008 Jan;307(1-2):73-82
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- COPE Antibody (C-term) (Cat. #AP51023PU-N) western blot analysis in A375 cell line lysates (35μg/lane).This demonstrates the COPE antibody detected the COPE protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of COPE Antibody (C-term)(Cat#AP51023PU-N) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- COPE Antibody (C-term) (Cat. #AP51023PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.