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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- STJ70058 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-BIF-1/SH3GLB1 antibody (C-Term) (STJ70058)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-BIF-1/SH3GLB1 (C-Term) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
QKGKVPITYLELLN- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70058-P1 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70058 staining (0. 1µg/ml) of HEK293 (A) and A431 (B) cell lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70058 staining (0. 1µg/ml) of NIH3T3 (A) , RAW264. 7 (B) and KNRK (C) cell lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
