HPA014305

antibody from Atlas Antibodies

Targeting: MYOM1

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA014305

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA014305, RRID:AB_1854257

Product name

Anti-MYOM1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

GITDTEEERIKEAAAYIAQRNLLASEEGITTPKQS
TASKQTTASKQSTASKQSTASKQSTASRQSTASRQ
SVVSKQATSALQQEETSEKKSRKVVIREKAERLSL
RKTLEETETYHAKLNEDHLLHAPEFIIKPRSHTVW
EKENV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human heart muscle and pancreas tissues using HPA014305 antibody. Corresponding MYOM1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human heart muscle, pancreas, prostate and skeletal muscle using Anti-MYOM1 antibody HPA014305 (A) shows similar protein distribution across tissues to independent antibody HPA049193 (B).

Antibody #2 product nr

HPA049193

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-MYOM1 antibody HPA014305.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-MYOM1 antibody HPA014305.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-MYOM1 antibody HPA014305.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle using Anti-MYOM1 antibody HPA014305.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells and islets of Langerhans as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows no positivity in smooth muscle cells as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Sample type

HUMAN