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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NB300-171 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-171, RRID:AB_10001651
- Product name
- Rabbit Polyclonal GluR1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is s completely eliminated by treatment with lambda-Ptase.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.15 ml
- Concentration
- 0.06 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Memory recall and modifications by activating neurons with elevated CREB.
Kim J, Kwon JT, Kim HS, Josselyn SA, Han JH
Nature neuroscience 2014 Jan;17(1):65-72
Nature neuroscience 2014 Jan;17(1):65-72
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: GluR1 [p Ser845] Antibody [NB300-171] - Rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
