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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PPS008 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-GluR1 (S845) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Specific for the ~100 kDa GluR1 protein phosphorylated at S845 in Western blots
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 150 uL
- Storage
- For long-term storage,
Submitted references Dopamine-dependent effects on basal and glutamate stimulated network dynamics in cultured hippocampal neurons.
Li Y, Chen X, Dzakpasu R, Conant K
Journal of neurochemistry 2017 Feb;140(4):550-560
Journal of neurochemistry 2017 Feb;140(4):550-560
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Phospho-GluR1 (S845) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at S845 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-GluR1 (S845). The immunolabeling is completely eliminated by treatment with lambda-PPase.
