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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-21791 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Molt-4, GL261, BCL-1, Raw264.7, PC-12.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAF1 using A) 30 µg GL261 whole cell lysate (B) 30 µg BCL-1 whole cell lysate and C) 30 µg Raw264.7 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MAF1 polyclonal antibody (Product # PA5-21791) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAF1 using 30 µg PC-12 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MAF1 polyclonal antibody (Product # PA5-21791) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAF1 using 30 µg of A) A431 and B) MOLT4 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MAF1 polyclonal antibody (Product # PA5-21791) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAF1 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAF1 Polyclonal Antibody (Product # PA5-21791) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MAF1 in paraformaldehyde-fixed HeLa cells using a MAF1 polyclonal antibody (Product # PA5-21791) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 (Product # PA5-21791) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAF1 Polyclonal Antibody detects MAF1 protein at nucleus and cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. MAF1 Polyclonal Antibody (Product # PA5-21791) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAF1 Polyclonal Antibody detects MAF1 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. MAF1 Polyclonal Antibody (Product # PA5-21791) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
