NBP1-19128

antibody from Novus Biologicals

Targeting: LARP1 KIAA0731, Lar1, LARP, Lhp1, MGC19556

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-19128

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-19128, RRID:AB_1642503

Product name

Rabbit Polyclonal LARP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

LARP1 binding to hepatitis C virus particles is correlated with intracellular retention of viral infectivity.

Plissonnier ML, Cottarel J, Piver E, Kullolli M, Centonze FG, Pitteri S, Farhan H, Meunier JC, Zoulim F, Parent R
Virus research 2019 Oct 2;271:197679

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Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus.

Plissonnier ML, Lahlali T, Michelet M, Lebossé F, Cottarel J, Beer M, Neveu G, Durantel D, Bartosch B, Accardi R, Clément S, Paradisi A, Devouassoux-Shisheboran M, Einav S, Mehlen P, Zoulim F, Parent R
PLoS biology 2016 Mar;14(3):e1002421

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The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition.

Moldovan JB, Moran JV
PLoS genetics 2015 May;11(5):e1005121

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LARP1 Antibody [NBP1-19128] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LARP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: LARP1 Antibody [NBP1-19128] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LARP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: LARP1 Antibody [NBP1-19128] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 antibody used for IP at 6 ug per reaction. LARP1 was also immunoprecipitated by rabbit anti-LARP1 antibody A302-088A. For blotting immunoprecipitated LARP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: LARP1 Antibody [NBP1-19128] - Detection of human LARP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LARP1 NBP1-19128 used for IP at 3 ug per reaction. LARP1 was also immunoprecipitated by rabbit anti-LARP1 antibody NBP1-19129. For blotting immunoprecipitated LARP1, NBP1-19128 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LARP1 Antibody [NBP1-19128] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-LARP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: LARP1 Antibody [NBP1-19128] - Human colon carcinoma. Antibody: Affinity purified rabbit anti-LARP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB