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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX81874 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81874, RRID:AB_11162289
- Product name
- CNOT8 antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CNOT8 Antibody (C-term) (GTX81874) western blot analysis in U251 cell line lysates (35μg/lane).This demonstrates the CNOT8 antibody detected the CNOT8 protein (arrow).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent confocal image of Hela cell stained with CNOT8 Antibody (C-term)(GTX81874).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term)(GTX81874), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram)(GTX81874) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
