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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [7]
- Immunohistochemistry [1]
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- Product number
- PA5-30789 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYL7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, mouse heart, rat heart.
- Concentration
- 3.11 mg/mL
Submitted references Single-cell protein expression of hiPSC-derived cardiomyocytes using Single-Cell Westerns.
Jabart E, Molho J, Sin K, Stansfield B, Kazmouz SG, Ventro D, Gardner K, Wu JC, Churko JM
Journal of molecular and cellular cardiology 2020 Dec;149:115-122
Journal of molecular and cellular cardiology 2020 Dec;149:115-122
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYL7 using 30 µg of A) A431 and B) HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MYL7 polyclonal antibody (Product # PA5-30789) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYL7 using 50 µg of mouse heart lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MYL7 polyclonal antibody (Product # PA5-30789) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Brain (Lane 3) and Rat Brain (Lane 4). The blot was probed with Anti-MYL7 Polyclonal Antibody (Product # PA5-30769, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 19kDa band corresponding to MYL7 was observed in Heart tissues except Brain tissues which are reported to be negative.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MYL7 was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a MYL7 Polyclonal Antibody (Product # PA5-30789) at a dilution of 1:150,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MYL7 Polyclonal Antibody (Product # PA5-30789). Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with MYL7 Polyclonal Antibody (Product # PA5-30789) diluted at 1:100,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MYL7 Polyclonal Antibody (Product # PA5-30789). Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with MYL7 Polyclonal Antibody (Product # PA5-30789) diluted at 1:100,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MYL7 Polyclonal Antibody detects MYL7 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MYL7 Polyclonal Antibody (Product # PA5-30789) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using MYL7 (Product # PA5-30789) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
