PA5-117945

antibody from Invitrogen Antibodies

Targeting: FADS3 CYB5RP, LLCDL3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117945

Provider

Invitrogen Antibodies

Product name

FADS3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of FADS3 in Lane A: Rat kidney tissue lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117945) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 51 kDa, Observed band size: 51 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FADS3 Polyclonal Antibody (Product # PA5-117945) at 1:500 dilution. Lane A: Rat kidney tissue lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 51 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FADS3 Immunoprecipitation using: Lane A: 0.5 mg Rat kidney Whole Cell Lysate 4 µL with FADS3 Polyclonal Antibody (Product # PA5-117945) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: FADS3 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 54 kDa.