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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [23]
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- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00009927-M03 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Mitofusin 2 Antibody
- Antibody type
- Monoclonal
- Description
- Ascites.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mitochondrial Dynamin-Related Protein 1 (DRP1) translocation in response to cerebral glucose is impaired in a rat model of early alteration in hypothalamic glucose sensing.
Acquired Expression of Mutant Mitofusin 2 Causes Progressive Neurodegeneration and Abnormal Behavior.
Inhibiting neddylation modification alters mitochondrial morphology and reprograms energy metabolism in cancer cells.
Acetylation as a major determinant to microtubule-dependent autophagy: Relevance to Alzheimer's and Parkinson disease pathology.
Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males.
The thiol switch C684 in Mitofusin-2 mediates redox-induced alterations of mitochondrial shape and respiration.
A new mitofusin topology places the redox-regulated C terminus in the mitochondrial intermembrane space.
Regulation of ER-mitochondria contacts by Parkin via Mfn2.
The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.
Dietary supplementation with acetyl-l-carnitine counteracts age-related alterations of mitochondrial biogenesis, dynamics and antioxidant defenses in brain of old rats.
Physiological, biochemical, anthropometric, and biomechanical influences on exercise economy in humans.
Mitochondrial Metabolism Power SIRT2-Dependent Deficient Traffic Causing Alzheimer's-Disease Related Pathology.
Mitophagy receptor FUNDC1 regulates mitochondrial dynamics and mitophagy.
Mitochondrial dysfunction in hereditary spastic paraparesis with mutations in DDHD1/SPG28.
Twenty-eight days of exposure to 3454 m increases mitochondrial volume density in human skeletal muscle.
DRP1-dependent apoptotic mitochondrial fission occurs independently of BAX, BAK and APAF1 to amplify cell death by BID and oxidative stress.
The Upshot of LRRK2 Inhibition to Parkinson's Disease Paradigm.
Exercise training can induce cardiac autophagy at end-stage chronic conditions: insights from a graft-versus-host-disease mouse model.
Over-expressing mitofusin-2 in healthy mature mammalian skeletal muscle does not alter mitochondrial bioenergetics.
Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training.
Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study.
Mitochondrial bioenergetics and dynamics interplay in complex I-deficient fibroblasts.
PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta protein.
Desmoulins L, Chrétien C, Paccoud R, Collins S, Cruciani-Guglielmacci C, Galinier A, Liénard F, Quinault A, Grall S, Allard C, Fenech C, Carneiro L, Mouillot T, Fournel A, Knauf C, Magnan C, Fioramonti X, Pénicaud L, Leloup C
Molecular metabolism 2019 Feb;20:166-177
Molecular metabolism 2019 Feb;20:166-177
Acquired Expression of Mutant Mitofusin 2 Causes Progressive Neurodegeneration and Abnormal Behavior.
Ishikawa K, Yamamoto S, Hattori S, Nishimura N, Tani H, Mito T, Matsumoto H, Miyakawa T, Nakada K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Feb 27;39(9):1588-1604
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Feb 27;39(9):1588-1604
Inhibiting neddylation modification alters mitochondrial morphology and reprograms energy metabolism in cancer cells.
Zhou Q, Li H, Li Y, Tan M, Fan S, Cao C, Meng F, Zhu L, Zhao L, Guan MX, Jin H, Sun Y
JCI insight 2019 Feb 21;4(4)
JCI insight 2019 Feb 21;4(4)
Acetylation as a major determinant to microtubule-dependent autophagy: Relevance to Alzheimer's and Parkinson disease pathology.
Esteves AR, Palma AM, Gomes R, Santos D, Silva DF, Cardoso SM
Biochimica et biophysica acta. Molecular basis of disease 2019 Aug 1;1865(8):2008-2023
Biochimica et biophysica acta. Molecular basis of disease 2019 Aug 1;1865(8):2008-2023
Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males.
Larsen S, Lundby AM, Dandanell S, Oberholzer L, Keiser S, Andersen AB, Haider T, Lundby C
Physiological reports 2018 Sep;6(18):e13793
Physiological reports 2018 Sep;6(18):e13793
The thiol switch C684 in Mitofusin-2 mediates redox-induced alterations of mitochondrial shape and respiration.
Thaher O, Wolf C, Dey PN, Pouya A, Wüllner V, Tenzer S, Methner A
Neurochemistry international 2018 Jul;117:167-173
Neurochemistry international 2018 Jul;117:167-173
A new mitofusin topology places the redox-regulated C terminus in the mitochondrial intermembrane space.
Mattie S, Riemer J, Wideman JG, McBride HM
The Journal of cell biology 2018 Feb 5;217(2):507-515
The Journal of cell biology 2018 Feb 5;217(2):507-515
Regulation of ER-mitochondria contacts by Parkin via Mfn2.
Basso V, Marchesan E, Peggion C, Chakraborty J, von Stockum S, Giacomello M, Ottolini D, Debattisti V, Caicci F, Tasca E, Pegoraro V, Angelini C, Antonini A, Bertoli A, Brini M, Ziviani E
Pharmacological research 2018 Dec;138:43-56
Pharmacological research 2018 Dec;138:43-56
The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.
Zhao YG, Chen Y, Miao G, Zhao H, Qu W, Li D, Wang Z, Liu N, Li L, Chen S, Liu P, Feng D, Zhang H
Molecular cell 2017 Sep 21;67(6):974-989.e6
Molecular cell 2017 Sep 21;67(6):974-989.e6
Dietary supplementation with acetyl-l-carnitine counteracts age-related alterations of mitochondrial biogenesis, dynamics and antioxidant defenses in brain of old rats.
Nicassio L, Fracasso F, Sirago G, Musicco C, Picca A, Marzetti E, Calvani R, Cantatore P, Gadaleta MN, Pesce V
Experimental gerontology 2017 Nov;98:99-109
Experimental gerontology 2017 Nov;98:99-109
Physiological, biochemical, anthropometric, and biomechanical influences on exercise economy in humans.
Lundby C, Montero D, Gehrig S, Andersson Hall U, Kaiser P, Boushel R, Meinild Lundby AK, Kirk N, Valdivieso P, Flück M, Secher NH, Edin F, Hein T, Madsen K
Scandinavian journal of medicine & science in sports 2017 Dec;27(12):1627-1637
Scandinavian journal of medicine & science in sports 2017 Dec;27(12):1627-1637
Mitochondrial Metabolism Power SIRT2-Dependent Deficient Traffic Causing Alzheimer's-Disease Related Pathology.
Silva DF, Esteves AR, Oliveira CR, Cardoso SM
Molecular neurobiology 2017 Aug;54(6):4021-4040
Molecular neurobiology 2017 Aug;54(6):4021-4040
Mitophagy receptor FUNDC1 regulates mitochondrial dynamics and mitophagy.
Chen M, Chen Z, Wang Y, Tan Z, Zhu C, Li Y, Han Z, Chen L, Gao R, Liu L, Chen Q
Autophagy 2016;12(4):689-702
Autophagy 2016;12(4):689-702
Mitochondrial dysfunction in hereditary spastic paraparesis with mutations in DDHD1/SPG28.
Mignarri A, Rubegni A, Tessa A, Stefanucci S, Malandrini A, Cardaioli E, Meschini MC, Stromillo ML, Doccini S, Federico A, Santorelli FM, Dotti MT
Journal of the neurological sciences 2016 Mar 15;362:287-91
Journal of the neurological sciences 2016 Mar 15;362:287-91
Twenty-eight days of exposure to 3454 m increases mitochondrial volume density in human skeletal muscle.
Jacobs RA, Lundby AK, Fenk S, Gehrig S, Siebenmann C, Flück D, Kirk N, Hilty MP, Lundby C
The Journal of physiology 2016 Mar 1;594(5):1151-66
The Journal of physiology 2016 Mar 1;594(5):1151-66
DRP1-dependent apoptotic mitochondrial fission occurs independently of BAX, BAK and APAF1 to amplify cell death by BID and oxidative stress.
Oettinghaus B, D'Alonzo D, Barbieri E, Restelli LM, Savoia C, Licci M, Tolnay M, Frank S, Scorrano L
Biochimica et biophysica acta 2016 Aug;1857(8):1267-1276
Biochimica et biophysica acta 2016 Aug;1857(8):1267-1276
The Upshot of LRRK2 Inhibition to Parkinson's Disease Paradigm.
Esteves AR, G-Fernandes M, Santos D, Januário C, Cardoso SM
Molecular neurobiology 2015 Dec;52(3):1804-1820
Molecular neurobiology 2015 Dec;52(3):1804-1820
Exercise training can induce cardiac autophagy at end-stage chronic conditions: insights from a graft-versus-host-disease mouse model.
Fiuza-Luces C, Delmiro A, Soares-Miranda L, González-Murillo Á, Martínez-Palacios J, Ramírez M, Lucia A, Morán M
Brain, behavior, and immunity 2014 Jul;39:56-60
Brain, behavior, and immunity 2014 Jul;39:56-60
Over-expressing mitofusin-2 in healthy mature mammalian skeletal muscle does not alter mitochondrial bioenergetics.
Lally JS, Herbst EA, Matravadia S, Maher AC, Perry CG, Ventura-Clapier R, Holloway GP
PloS one 2013;8(1):e55660
PloS one 2013;8(1):e55660
Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training.
Russell AP, Lamon S, Boon H, Wada S, Güller I, Brown EL, Chibalin AV, Zierath JR, Snow RJ, Stepto N, Wadley GD, Akimoto T
The Journal of physiology 2013 Sep 15;591(18):4637-53
The Journal of physiology 2013 Sep 15;591(18):4637-53
Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study.
Cosson P, Marchetti A, Ravazzola M, Orci L
PloS one 2012;7(9):e46293
PloS one 2012;7(9):e46293
Mitochondrial bioenergetics and dynamics interplay in complex I-deficient fibroblasts.
Morán M, Rivera H, Sánchez-Aragó M, Blázquez A, Merinero B, Ugalde C, Arenas J, Cuezva JM, Martín MA
Biochimica et biophysica acta 2010 May;1802(5):443-53
Biochimica et biophysica acta 2010 May;1802(5):443-53
PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta protein.
Holloway GP, Perry CG, Thrush AB, Heigenhauser GJ, Dyck DJ, Bonen A, Spriet LL
American journal of physiology. Endocrinology and metabolism 2008 Jun;294(6):E1060-9
American journal of physiology. Endocrinology and metabolism 2008 Jun;294(6):E1060-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 over-expressed 293 cell line, cotransfected with MFN2 Validated Chimera RNAi ( Cat # H00009927-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN2 monoclonal antibody (M03), clone 4H8 (Cat # H00009927-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in transfected 293T cell line by MFN2 monoclonal antibody (M03), clone 4H8. Lane 1: MFN2 transfected lysatE (86.4 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in PC-12 (Cat # L012V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Detection limit for recombinant GST tagged MFN2 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 5 ug/ml
