NB100-57086

antibody from Novus Biologicals

Targeting: DGAT1 ARGP1, DGAT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-57086

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-57086, RRID:AB_843835

Product name

Goat Polyclonal DGAT1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Goat

Antigen sequence

QNSMKPFKDMDYS


Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Analysis of the liver lipidome reveals insights into the protective effect of exercise on high-fat diet-induced hepatosteatosis in mice.

Jordy AB, Kraakman MJ, Gardner T, Estevez E, Kammoun HL, Weir JM, Kiens B, Meikle PJ, Febbraio MA, Henstridge DC
American journal of physiology. Endocrinology and metabolism 2015 May 1;308(9):E778-91

---

Lipidomic and spatio-temporal imaging of fat by mass spectrometry in mice duodenum during lipid digestion.

Seyer A, Cantiello M, Bertrand-Michel J, Roques V, Nauze M, Bézirard V, Collet X, Touboul D, Brunelle A, Coméra C
PloS one 2013;8(4):e58224

---

Caspase-1 deficiency in mice reduces intestinal triglyceride absorption and hepatic triglyceride secretion.

van Diepen JA, Stienstra R, Vroegrijk IO, van den Berg SA, Salvatori D, Hooiveld GJ, Kersten S, Tack CJ, Netea MG, Smit JW, Joosten LA, Havekes LM, van Dijk KW, Rensen PC
Journal of lipid research 2013 Feb;54(2):448-56

---

Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance: another paradox in endurance-trained athletes?

Amati F, Dubé JJ, Alvarez-Carnero E, Edreira MM, Chomentowski P, Coen PM, Switzer GE, Bickel PE, Stefanovic-Racic M, Toledo FG, Goodpaster BH
Diabetes 2011 Oct;60(10):2588-97

---

Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training.

Alsted TJ, Nybo L, Schweiger M, Fledelius C, Jacobsen P, Zimmermann R, Zechner R, Kiens B
American journal of physiology. Endocrinology and metabolism 2009 Mar;296(3):E445-53

---

Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts.

Bagnato C, Igal RA
The Journal of biological chemistry 2003 Dec 26;278(52):52203-11

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Rat Duodenum lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DGAT1 Antibody [NB100-57086] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DGAT1 Antibody [NB100-57086] - Staining of paraffin embedded Human Colon. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: DGAT1 Antibody [NB100-57086] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.