AF7208

antibody from R&D Systems

Targeting: FTO ALKBH9, KIAA1752, MGC5149

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7208

Provider

R&D Systems

Product name

Human FTO Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human FTO in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q9C0B1


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Kaposi's Sarcoma-Associated Herpesvirus Utilizes and Manipulates RNA N⁶-Adenosine Methylation To Promote Lytic Replication.

Ye F, Chen ER, Nilsen TW
Journal of virology 2017 Aug 15;91(16)

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FTO by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, A172 human glioblastoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and HEC-1-B human endometrial adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FTO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7208) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FTO at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.