27146-1-AP

antibody from Invitrogen Antibodies

Targeting: NEK1 KIAA1901, NY-REN-55

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

27146-1-AP

Provider

Invitrogen Antibodies

Product name

NEK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KRKEAYERE KKVWEEHLVA KGVKSSDVSP PLGQHETGGS PSKQQMRSVI SVTSALKEVG VDSSLTDTRE TSEEMQKTNN AISSKREILR RLNENLKAQE DEKGKQNLSD TFEINVHEDA KEHEKEKSVS SDRKKWEAGG QLVIPLDELT LDTSFSTTER HTVGEVIKLG PNGSPRRAWG KSPTD (557-740 aa encoded by BC114491)

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-12 cells were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NEK1 antibody (Product # 27146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NEK1 (IP:27146-1-AP, 4ug; Detection:27146-1-AP 1:500) with PC-12 cells lysate 3080 ug.