Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1179 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat RAGE Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat RAGE in Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant canine RAGE is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O35444
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references RAGE is a Critical Mediator of Pulmonary Oxidative Stress, Alveolar Macrophage Activation and Emphysema in Response to Cigarette Smoke.
Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke.
Deletion of receptor for advanced glycation end products exacerbates lymphoproliferative syndrome and lupus nephritis in B6-MRL Fas lpr/j mice.
Tumor necrosis factor-α blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
Spermatogenic and sperm quality differences in an experimental model of metabolic syndrome and hypogonadal hypogonadism.
Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
Type I epithelial cells are the main target of whole-body hypoxic preconditioning in the lung.
Neural-activity-dependent release of S100B from astrocytes enhances kainate-induced gamma oscillations in vivo.
Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
Cutting edge: extracellular high mobility group box-1 protein is a proangiogenic cytokine.
Cutting edge: extracellular high mobility group box-1 protein is a proangiogenic cytokine.
Sanders KA, Delker DA, Huecksteadt T, Beck E, Wuren T, Chen Y, Zhang Y, Hazel MW, Hoidal JR
Scientific reports 2019 Jan 18;9(1):231
Scientific reports 2019 Jan 18;9(1):231
Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke.
Wolf L, Herr C, Niederstraßer J, Beisswenger C, Bals R
PloS one 2017;12(7):e0180092
PloS one 2017;12(7):e0180092
Deletion of receptor for advanced glycation end products exacerbates lymphoproliferative syndrome and lupus nephritis in B6-MRL Fas lpr/j mice.
Goury A, Meghraoui-Kheddar A, Belmokhtar K, Vuiblet V, Ortillon J, Jaisson S, Devy J, Le Naour R, Tabary T, Cohen JH, Schmidt AM, Rieu P, Touré F
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3612-22
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3612-22
Tumor necrosis factor-α blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M
Immunity 2013 Mar 21;38(3):541-54
Immunity 2013 Mar 21;38(3):541-54
Spermatogenic and sperm quality differences in an experimental model of metabolic syndrome and hypogonadal hypogonadism.
Mallidis C, Czerwiec A, Filippi S, O'Neill J, Maggi M, McClure N
Reproduction (Cambridge, England) 2011 Jul;142(1):63-71
Reproduction (Cambridge, England) 2011 Jul;142(1):63-71
Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
Yamakawa N, Uchida T, Matthay MA, Makita K
American journal of physiology. Lung cellular and molecular physiology 2011 Apr;300(4):L516-25
American journal of physiology. Lung cellular and molecular physiology 2011 Apr;300(4):L516-25
Type I epithelial cells are the main target of whole-body hypoxic preconditioning in the lung.
Zhang SX, Miller JJ, Stolz DB, Serpero LD, Zhao W, Gozal D, Wang Y
American journal of respiratory cell and molecular biology 2009 Mar;40(3):332-9
American journal of respiratory cell and molecular biology 2009 Mar;40(3):332-9
Neural-activity-dependent release of S100B from astrocytes enhances kainate-induced gamma oscillations in vivo.
Sakatani S, Seto-Ohshima A, Shinohara Y, Yamamoto Y, Yamamoto H, Itohara S, Hirase H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Oct 22;28(43):10928-36
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Oct 22;28(43):10928-36
Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM
The Journal of clinical investigation 2008 Jan;118(1):183-94
The Journal of clinical investigation 2008 Jan;118(1):183-94
Cutting edge: extracellular high mobility group box-1 protein is a proangiogenic cytokine.
Mitola S, Belleri M, Urbinati C, Coltrini D, Sparatore B, Pedrazzi M, Melloni E, Presta M
Journal of immunology (Baltimore, Md. : 1950) 2006 Jan 1;176(1):12-5
Journal of immunology (Baltimore, Md. : 1950) 2006 Jan 1;176(1):12-5
Cutting edge: extracellular high mobility group box-1 protein is a proangiogenic cytokine.
Mitola S, Belleri M, Urbinati C, Coltrini D, Sparatore B, Pedrazzi M, Melloni E, Presta M
Journal of immunology (Baltimore, Md. : 1950) 2006 Jan 1;176(1):12-5
Journal of immunology (Baltimore, Md. : 1950) 2006 Jan 1;176(1):12-5
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RAGE at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RAGE in Mouse Brain. RAGE was detected in immersion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.