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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [9]
- Flow cytometry [1]
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- Product number
- GTX84007 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84007, RRID:AB_10730135
- Product name
- NRBP1 antibody [5C3]
- Antibody type
- Monoclonal
- Reactivity
- Human, Simian
- Host
- Mouse
Submitted references NRBP1 is downregulated in breast cancer and NRBP1 overexpression inhibits cancer cell proliferation through Wnt/β-catenin signaling pathway.
Wei H, Wang H, Ji Q, Sun J, Tao L, Zhou X
OncoTargets and therapy 2015;8:3721-30
OncoTargets and therapy 2015;8:3721-30
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-NRBP1monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-NRBP1 mouse monoclonal antibody (GTX84007) immunofluorescent staining of COS7 cells transiently transfected with NRBP1
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NRBP1mouse monoclonal antibody. (GTX84007, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue using anti-NRBP1 mouse monoclonal antibody. (GTX84007, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-NRBP1mouse monoclonal antibody. (GTX84007, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-NRBP1 mouse monoclonal antibody. (GTX84007, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-NRBP1mouse monoclonal antibody. (GTX84007, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-NRBP1mouse monoclonal antibody. (GTX84007, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue using anti-NRBP1mouse monoclonal antibody. (GTX84007, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lymphoma tissue using anti-NRBP1mouse monoclonal antibody. (GTX84007, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-NRBP1mouse monoclonal antibody. (GTX84007, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either NRBP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody (GTX84007), and then analyzed by flow cytometry.
