LS-C185741

antibody from LSBio

Targeting: BUD23 MERM1, MGC19709, MGC2022, MGC5140, PP3381, WBMT, WBSCR22

Western blot (Enhanced validation)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C185741

Provider

LSBio

Product name

WBSCR22 Antibody (aa1-263) LS-C185741

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: HeLa, B: HCT116. 12% SDS PAGE. WBSCR22 antibody diluted at 1:1000.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 ug of WBSCR22 antibody 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 ug of WBSCR22 antibody 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 ug of WBSCR22 antibody 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded CA922 xenograft using WBSCR22 antibody at 1:100 dilution.