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LearnGTX105840
antibody from GeneTex
Targeting: BUD23
MERM1, MGC19709, MGC2022, MGC5140, PP3381, WBMT, WBSCR22
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX105840 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105840, RRID:AB_1952564
- Product name
- WBSCR22 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Natural killer cells inhibit oxaliplatin-resistant colorectal cancer by repressing WBSCR22 via upregulating microRNA-146b-5p.
The novel metastasis promoter Merm1/Wbscr22 enhances tumor cell survival in the vasculature by suppressing Zac1/p53-dependent apoptosis.
Zhao H, Su W, Kang Q, Xing Z, Lin X, Wu Z
American journal of cancer research 2018;8(5):824-834
American journal of cancer research 2018;8(5):824-834
The novel metastasis promoter Merm1/Wbscr22 enhances tumor cell survival in the vasculature by suppressing Zac1/p53-dependent apoptosis.
Nakazawa Y, Arai H, Fujita N
Cancer research 2011 Feb 1;71(3):1146-55
Cancer research 2011 Feb 1;71(3):1146-55
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa B: HCT116 12% SDS PAGE GTX105840 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WBSCR22 antibody detects WBSCR22 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: WBSCR22 protein stained by WBSCR22 antibody (GTX105840) diluted at 1:200.Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WBSCR22 antibody immunoprecipitates WBSCR22 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of WBSCR22 protein by 2.5 £gg of WBSCR22 antibody (GTX105840) 12% SDS-PAGE The immunoprecipitated WBSCR22 protein was detected by WBSCR22 antibody (GTX105840) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using WBSCR22 (GTX105840) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WBSCR22 antibody detects WBSCR22 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. WBSCR22 antibody (GTX105840) diluted at 1:500.
