MAB4920
antibody from Novus Biologicals
Targeting: CES1
CEH, CES1A1, CES1A2, CES2, HMSE, HMSE1, SES1
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
Reference
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- Product number
- MAB4920 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Carboxylesterase 1/CES1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Carboxylesterase1/CES-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) CES-3 and no cross-reactivity with rmCES-2, rmCES-5, or recombinant human CES-2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Human leukocytes differentially express endocannabinoid-glycerol lipases and hydrolyze 2-arachidonoyl-glycerol and its metabolites from the 15-lipoxygenase and cyclooxygenase pathways.
Turcotte C, Dumais É, Archambault AS, Martin C, Blanchet MR, Bissonnette É, Boulet LP, Laviolette M, Di Marzo V, Flamand N
Journal of leukocyte biology 2019 Dec;106(6):1337-1347
Journal of leukocyte biology 2019 Dec;106(6):1337-1347
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Carboxylesterase 1/CES1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Carboxylesterase 1/CES1 Monoclonal Antibody (Catalog # MAB4920) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carboxylesterase 1/CES1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Carboxylesterase 1/CES1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Carboxylesterase 1/CES1 at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Carboxylesterase 1/CES1 Monoclonal Antibody (Catalog # MAB4920). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.