Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [9]
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Validation data
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- Product number
- NBP1-81592 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-81592, RRID:AB_11028105
- Product name
- Rabbit Polyclonal HIP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat HIP1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration.
Majeed SR, Vasudevan L, Chen CY, Luo Y, Torres JA, Evans TM, Sharkey A, Foraker AB, Wong NM, Esk C, Freeman TA, Moffett A, Keen JH, Brodsky FM
Nature communications 2014 May 23;5:3891
Nature communications 2014 May 23;5:3891
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIP1 Antibody [NBP1-81592] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIP1 Antibody [NBP1-81592] - Analysis in human cell lines A-549 and Caco-2 using Anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Analysis in human testis and liver tissues using Anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining in human testis and liver tissues using anti-HIP1 antibody. Corresponding HIP1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human colon, kidney, liver and testis using Anti-HIP1 antibody NBP1-81592 (A) shows similar protein distribution across tissues to independent antibody NBP1-81593 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human testis using Anti-HIP1 antibody NBP1-81592.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIP1 Antibody [NBP1-81592] - Staining of human liver using Anti-HIP1 antibody NBP1-81592.