MA5-38539

antibody from Invitrogen Antibodies

Targeting: HLA-DRA HLA-DRA1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38539

Provider

Invitrogen Antibodies

Product name

HLA-DRA Monoclonal Antibody (1C11A5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody has been tested in indirect ELISA.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1C11A5

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HLA-DRA using HLA-DRA Monoclonal Antibody (Product # MA5-38539) in Ramos (1), and Raji (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis using HLA-DRA Monoclonal Antibody (Product # MA5-38539) against human HLA-DRA (AA: 26-254) recombinant protein. (Expected MW is 51.5 kDa)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HLA-DRA using HLA-DRA Monoclonal Antibody (Product # MA5-38539) in HEK293 (1) and HLA-DRA (AA: 26-254)-human IgG-Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of HLA-DRA in HeLa cells using HLA-DRA Monoclonal Antibody (Product # MA5-38539) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of HLA-DRA in MCF-7 cells using HLA-DRA Monoclonal Antibody (Product # MA5-38539) (green) and negative control (red).