14069-1-AP

antibody from Invitrogen Antibodies

Targeting: INTS5 INT5, KIAA1698

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14069-1-AP

Provider

Invitrogen Antibodies

Product name

INTS5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSALCDPPG APGPPGPAPA THGPAPLSAQ ELSQEIKAFL TGVDPILGHQ LSAREHARCG LLLLRSLPPA RAAVLDHLRG VFDESVRAHL AALDETPVAG PPHLRPPPPS HVPAGGPGLE DVVQEVQQVL SEFIRANPKA WAPVISAWSI DLMGQLSSTY SGQHQRVPHA TGALNELLQL WMGCRATRTL MDIYVQCLSA LIGSCPDACV DALLDTSVQH SPHFDWVVAH IGSSFPGTII SRVLSCGLKD FCVHGGAGGG AGSSGGSSSQ TPSTDPFPGS PAIPAEKRVP KIASVVGILG HLASRHGDSI RRELLRMFHD SLA (1-322 aa encoded by BC060841)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14069-1-AP (INTS5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 14069-1-AP (INTS5 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 14069-1-AP (INTS5 antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-INTS5 (IP:14069-1-AP, 4ug; Detection:14069-1-AP 1:1000) with HeLa cells lysate 2000ug.