PA5-28535

antibody from Invitrogen Antibodies

Targeting: ASB5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28535

Provider

Invitrogen Antibodies

Product name

ASB5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse muscle.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASB5 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASB5 Polyclonal Antibody (Product # PA5-28535) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ASB5 Polyclonal Antibody (Product # PA5-28535). Sample (30 µg whole cell lysate). A: A431. B: HeLa S3. C: Hep G2. 10% SDS PAGE. ASB5 Polyclonal Antibody (Product # PA5-28535) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ASB5 in methanol-fixed Hep G2 cells using an ASB5 polyclonal antibody (Product # PA5-28535) at a 1:500 dilution.